Root tip mitosis page - rtm6 4 based upon the class results, order the stages of mitosis from shortest (1) to longest (4) after the longest and shortest stage, give a brief explanation of why that stage may have that time period. Gtac mitosis in root tips page 3 of 4 method: staining and squash preparation wear safety glasses 1 the root tips have been grown and placed into fixative solution prior to class (min 2h fixation. An improved and simplified protocol for showing root-tip mitosis, using toluidine blue stain science and plants for schools (saps) - supporting plant science in uk secondary schools and post-16. Using garlic root tips to observe mitosis 1030 words oct 27th, 2011 5 pages mitosis is a cellular process that replicates chromosomes and produces two identical nuclei in preparation for cell division, from the original cell two cells are derived, each of which possesses the same genetic material.
Garlic root tips in fixative solution (fixative solution freezes the cell at whatever stage of mitosis the cell is undergoing when it is places into it) single razor blade monocular microscope. Procedure data results claim evidence reflection questions garlic root tip squash lab 1 the main thing that affect the rate of diffusion is what kind of cellists some cells go on in the cell cycle. After they have made the root tip slide, students will view their prepared slide under a light microscope at 400x they will methodically scan the slide counting 100 cells they will make note of the phase of mitosis each cell is in students will express that data as a proportion and then determine how long the cell is in each phase during a.
Mitosis is a cellular process that replicates chromosomes and produces two identical nuclei in preparation for cell division mitosis has five phases: interphase, prophase, metaphase, anaphase and telophase. Explain why the whitefish blastula and onion root tip are selected for a study of mitosis blastula and root tips are where the creation of new cells happens that is where the cells split and is where nuclear replication is the most frequent. Advance preparation to grow the root tips, garlic cloves need to be suspended over a beaker of water suitable roots will grow within three to ten days leaving them any longer than this will mean that cell growth will have slowed down and the chance of observing mitosis in cells will be very slim. In plants, cell division by mitosis is restricted to specific tissues, called meristems, that are found at the growing root and shoot tips and in the cambium between the xylem and phloem of the vascular bundle. This feature is not available right now please try again later.
I used this procedure on onion root tips and garlic root tips as well the one thing i found - the root tips are only in the various stages of mitosis at certain times of the day. The photomicrograph below depicts onion root tip cell chromosomes in metaphase, ready for separation the kinetochore and polar microtubules are clearly visible and radiate out the ends of the cell leaving the chromosomes in the middle of the complex. The time taken for a root tip to immerse in stains can be monitored by using a stop watch 4-5 mm of root tips are cut for the easy handling of the root tip and maceration moreover the root tip is macerated to ensure a single layer of root tip is obtained white. 1 mitosis in onion root tip cells a quick overview of cell division the genetic information of plants, animals and other eukaryotic organisms resides in several (or. The root tip meristem is usually a denser white and more rounded than the cut end chromosomes in root tip tissue are made visible with the stain dividing cells (if present) will show up clearly with chromosomes in different forms according to the stage of mitosis.
Mitosis in garlic root tips biology inheritance - mitotic squash of garlic root tip mitosis is the process of cell division it is the process by which a eukaryotic cell separates the chromosomes in its cell nucleus into two identical sets in two nuclei. Transfer 1 tip onto a microscope slide and cut 4-5mm from the growing tip (site of mitosis) and keep it gently break up (macerate) the root tip with a mounted needle add 1 small drop of orcein ethanoic stain and leave for 2 minutes. Mitosis in onion root tip cells replacing old cells and tissue repair for single-celled, and some multi-cellular eukaryotes, mitosis is part of asexual reproduction. The use of garlic roots to observe mitosis ,was a good choice as the stages in plant growth can be observed on the meristem , easier than animal cells as meristem is always dividing mitosis, every stage can be observed clearly.
This experiment use the garlic root tips tissue for observing the mitosis process because the stages of development in plant growth can be distinctly observed at this part which is known as meristem this meristem is actively divided by mitosis. Garlic is very reliable, but cell division in root tip meristems of onions, hyacinths and broad beans can also be observed using this protocol: information on growing these is given above references ofqual. Although slicing the onion root captures many cells in different phases of the cell cycle, keep in mind that the cell cycle is a continuous process scientists have divided the process into 5 phases, each characterized by important events, but these divisions are still arbitrary. The root tip should spread out to a diameter of about 05- 1cm observe it under a compound microscope in 10x objective scan and narrow down to a region containing dividing cells and switch to 40x for a better view.